A H2O2-free electrochemiluminescence immunosensor constructed on all-in-one bioprobe comprised of TiO2-B supported fluoro-coumarin silicon phthalocyanine for deoxynivalenol sensing

Abstract A highly sensitive electrochemiluminescence (ECL) immunosensor was fabricated for the quantitative analysis of deoxynivalenol (DON) based on an effective all-in-one bioprobe that composed of TiO 2 -B with Fluoro-coumarin silicon phthalocyanine (F-couSiPc) and luminol. Herein, TiO 2 -B with large specific area was served as a favorable carrier, greatly increased the loading of enhancer F-couSiPc and luminophore luminol, resulting in the tremendous enhancement of ECL signal. Specifically, F-couSiPc as coreaction accelerator could facilitate the decomposition of dissolved O 2 to generate substantial reactive oxygen species (ROS) to participate in the ECL reaction of luminol. Additionally, the prepared PEDOT/HA@mp MnO 2 was employed as the sensing platform of biosensor, which can not only capture substantial antibody to improve the sensitivity, but also could expedite the electronic transmission rate to make the ECL intensity further amplified. Ultimately, the proposed immunosensor presented favorable performance for the determination of DON, such as high sensitivity, good stability, wide liner range (from 1.0*10 −6 ng/mL to 10 ng/mL) and a low detection limit of 3.3*10 -7 ng/mL.