The detection of enteroviruses in sewage using Caco-2 cells.

A b s t r a c t !e work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic $occulation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more e"ective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.