A fusion method based on alignment software with SNP and Indel detection methods

Background With the advent of the second generation sequencing technology, the discovery of sequence alignment and sequence variation is a long-standing challenge. Results A method based on general alignment software, SNP and Indel software tools was proposed in this paper. By comparing the advantages of traditional alignment software, we can produce the best alignment. SNP and Indel detection tools fusion research found that different depth of sequencing effect is different. When the sequence depth reaches a certain value, select one of the software for testing. Conclusions Finally, the experimental verification shows that SNP and Indel have certain advantages in the comparison of the effects of the fusion method.