P04-12. Characterization of leukopak PBMC phenotypes and biotypes for optimal performance in HIV-1 neutralization assays

Methods Leukopaks (N = 51) were analyzed using multi-parametric flow cytometry (LSRII cytometer, Becton Dickinson) to quantitate immune cell populations and surface markers. Viral titrations were performed using infectious molecular clones (IMC) (293T cell-derived or PBMC-passaged) and IC50 values were calculated using the Spearman-Karber formula. Productive replication in leukopaks was analyzed by p24 or luciferase expression; phenotypic correlates of viral permissiveness were assessed by Spearman correlation analyses.