High-performance liquid chromatographic analysis of peptides on an asahipak gs-320 column packed with hydrophilic polymer gel

Abstract Investigation of the chromatographic characteristics of various amino acids and peptides on an Asahipak GS-320 column, developed for high-performance gel filtration chromatography, revealed that these substances, which possess hydrophobic sites, are retained by adsorption and eluted in order of increasing hydrophobicity. The use of organic solvents as eluents was studied and practical separations of amino acids and peptides by isocratic elution were obtained.