Impact Ionic Liquid [Emim]OAc to Cellulose Hydrolysis Activities of Indigenous Microorganisms (IMO’s) on Cassava Peels Substrate

An ionic liquid (IL), 1-ethyl-3-methylimidazolium acetate ([Emim]OAc), was used as pretreatment catalyst follow by hydrolysis of cellulose on cassava peels using local selected indigenous microorganisms (IMO’s). Under 25% (w/v) loading mass of substrate and liquid fermentation condition, cellulolytic of cellulase activities and growth of IMO’s were investigated under a series of IL concentration. Optimal temperature and pH were 37C and 5.5 respectively. Enzyme activity was determined by analyzing the hydrolysis of carboxymethylcellulose (CMC) at 37C using the dinitrosalicylic acid. In general, 0.1 M of IL did not inhibit cell growth significantly. In addition, the hydrolytic activities of obtained-cellulases were most active in presence of 0.1 M of IL, while 0.5 M of IL pushed down the activity into an half and 1.0M extremely was losing the activity. The optimum experimental conditions for hydrolysis of pretreated cassava peels using 0.1 M of [Emim]OAc was established at 37 C, for 72 h when yield of glucose rose up to 64.49%.