The design of rapid MicroRNA detection system

In order to detect miRNA quickly, we designed a new portable device for the rapid detection of miRNA, using Opto-electronic detection technology, marking miRNA and isothermal rolling circle amplification and detecting markers which excite fluorescence intensity, the recognition system of characteristic fluorescence analysis was established. By changing the excitation light intensity, miRNA reagent concentration and other parameters, we arrive at the conclusion that there is the linear relationship (R2=0.9947) between miRNA concentration and fluorescence intensity when the miRNA concentration range the instrument can measure is in the range of 0.01-0.1mol and the lowest values measured by the instrument in theory is 7 copies.