Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex

Viola Maria Schmidt,Vladimir Isachenko,Gunter Rappl,Gohar Rahimi,Bettina Hanstein,B Morgenstern,P Mallmann,Evgenia Isachenko

Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex

2018

Viola Maria Schmidt
Vladimir Isachenko
Gunter Rappl
Gohar Rahimi
Bettina Hanstein
B Morgenstern
P Mallmann
Evgenia Isachenko

Background The aim of this study was to examine the effectiveness of Tumor Dissociation Enzyme (TDE) on the viability of follicles after digestion of fresh and cryopreserved ovarian cortex fragments (OCFs).

Keywords:

Enzyme
Digestion
Cryopreservation
Molecular biology
Ovarian Cortex
Follicle
Biology
Staining
Neutral red
Calcein AM

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