In silico and In vivo Toxicological Evaluation of Cissampelos Sympodialis Secondary Metabolites in Rattus Norvegicus

Cissampelos sympodialis is a plant to northeastern Brazil region used by populace for treating diseases respiratory. Several studies have shown that the ethanol extract of the leaves have immunomodulatory and anti-inflammatory activities. The infusion is an ancient popular technique widely used in traditional medicine using as extracting means just hot water. This study aims to investigate the acute toxicological potential administering a dose of 2000mg/kg in Rattus norvegicus combined with in silico study of 117 alkaloids present in Cissampelos genre. In silico, 5 we can determine which molecules have a high toxicity (21, 8, 93, 32 and 88) and low toxicity (57, 77, 28, 25 and 67) and their percentage metabolizing liver. The toxicological evaluation in vivo showed that there was a decrease in water consumption in males only and feed intake in both sexes. However, these numbers were not statistically sufficient to change the weight gain of the animals. As for the biochemical parameters of urea there was an increase and decrease of uric acid and AST in males. In females was a decrease in albumin and globulin which consequently leads to a decrease in total protein. Despite the biochemical changes are suggestive for kidney damage, histological sections confirm that there was no change in this organ and as well as in the liver. Therefore, the results indicate that despite the genus Cissampelos present alkaloids which may be toxic, the infusion of Cissampelos sympodialis sheets when applied orally at a dose of 2000mg/kg presents a low toxicity.