Simultaneous Determination of Five Components in Compound Preparation of Fuzheng Jiedu Keli by HPLC

Objective: To develop a HPLC method for determination of tanshinol,protocatechuic aldehyde,ferulic acid,salvianolic acid B,formononetin in compound preparation of Fuzheng Jiedu Keli.Method: The chromatographic separation was performed on a Dikmal ODS-C18 column(4.6 mm×250 mm,5 μm) maintaining at 30 ℃ during the whole process.The mobile phase consisted of methanol and 0.05%H3PO4 aqueous solution at a flow rate of 0.8 mL·min-1.The detection wavelength was set at 280 nm for tanshinol,protocatechuic aldehyde,salvianolic acid B,316 nm for ferulic acid,248 nm for formononetin.Result: All the compounds were linear(r0.999) in the range of the test concentrations,and the average recovery of the method was between 98% and 101%,RSD3.55%.Conclusion: The method is proved to be credible,sensitive,accurate and repeatable.It can be applied to determine tanshinol,protocatechuic aldehyde,salvianolic acid B,ferulic acid,formononetin in compound preparation of Fuzheng Jiedu Keli simultaneously and provide a basal method of quality control to this preparation and other relative preparations.