A comparative analysis of immunoenzyme methods of determining bradykinin

: Various modifications of enzyme immunoassay (EIA) for measuring kallikrein-kinin system components (bradykinin, kininogen, kallikrein) are considered. Bradykinin assays are analyzed in detail: double antibodies and competitive solid-phase EIA techniques with the use of various enzymic labels. Special attention is paid to the solid-phase variant in polymeric microplates, the simplest technique that holds good promise for clinical application. The authors have used this method for bradykinin measurements and tried to improve its sensitivity.