Myocardial protective effects of luteolin on isolated rat heart in hypothermic preservation

Objective: To investigate the protective effect of luteolin on isolated rat heart in hypothermic preservation.Methods: Forty male SD rats were randomly divided into 4 groups(n=10): control group,luteolin low-dose group(7.5 μmol/L),middle-dose group(15 μmol/L) and high dose group(30 μmol/L).Langendorff model of isolated rat heart was used.After 30 min basal perfusion,the hearts were stored in University of Wisconsin solution(UW solution) at 4°C with luteolin(7.5,15 and 30 μmol/L) or without luteolin for 12 h and followed by 60 min reperfusion.The recovery of cardiac contractile and diastolic function,coronary flow(CF),creatine kinase(CK) leakage in the coronary effluent,myocardial water content were determined.The myocardial ultrastructure was also observed.Results: The results revealed that luteolin improved the recovery of left ventricular peak systolic pressure and ±dp/dtmax dose-dependently and increased coronary flow.The leakage of creatine kinase in the coronary effluent was significantly reduced in luteolin-added hearts.Impairment of myocardial ultrastructure after 12 h hypothermic preservation was obviously alleviated in hearts luteolin-added group compared with that in control group.There were no differences between the groups in myocardial water contents.Conclusion: Luteolin as a supplementation in cardiac preservation solution can significantly improve the hypothermic preservation effects on rat heart and have myocardial protection effect,especially in luteolin-added with 30 μmol/L.