Descrição de transmissão horizontal e vertical de Porcine circovirus 2b (PCV2b) em camundongos (CH3/Rockfeller) inoculados experimentalmente

The studies of mice susceptibility to PCV2 infection are still controversial, despite the importance of PCV2 infection in pigs. Therefore, the aim of this study is to verify the occurrence of horizontal and vertical transmission of PCV2b in mice experimentally infected. Two experiments were performed. In experiment 1 an infected male mice covered a female to verify vertical infection. The pups were tested to detected viral DNA. In experiment 2 the 21 days of age mice were inoculated with PCV2 and allocated with uninfected, i.e. contactantes, to evaluate the horizontal infection. Subsequently, the contactants and inoculated mice were tested for PCV2 DNA. The samples collected from both experiments had their DNA extracted and were further submitted to polymerase chain reaction (PCR). In experiment 1, after the gestation period, the female farrowed 10 mices of which 60% (6/10) were positive for the agent. In experiment 2, both the inoculated and the contacts animals showed no clinical changes and gross lesions of PCV2 infection, but viral DNA was recovered in four of the five animals of the contactant group. The detection of viral DNA in the offspring of the primo-infected females and in the mice that had contact with inoculated mice demonstrated that PCV2 can be transmitted vertically and horizontally.