Cloning and analysis on tissue specific expression of a Actin gene fragment from Agropyron mongolicum

In this study,actin gene homology fragment from Mongo1ian wheatgrass(Agropyron mongolicum)was isolated using homology-based method,and analyzed on tissue specific expression of endogenous actin gene.The primers A1 was designed according to the amino acid conserved regions of the cloned actin gene of Gramineae plant wheat,one partials gene sequences have been obtained by reverse transcription polymerase chain reaction(RT-PCR).Sequence analysis using Software of DNAman and DNAuser etc,and showed that the cDNA sequences was 541 bp,encoding 179 amino acids.And putative conserved domains have been detected,including ACTIN superfamily domains of ATP binding site,profilin binding site and gelsolin binding site.Homology comparison with Actin gene amino acid sequences in other plants showed that it was 99% identical to wheat,96% to maize and 93% to rice.The results of analysis on tissue specific expression showed that the endogenous actin gene expression level in the root,stem and leaf of Mongo1ian wheatgrass was identical.It was named as MwACT1,GenBank accession number: FJ490410.