Amplification,Cloning and Bioinformatic Analysis of Porcine TLR7 Gene

One pair of primers was designed and synthesized according to the nucleic acid sequence of porcine toll-like receptor 7(TLR7)gene.The TLR7 gene from Sanyuan pig was amplified by RT-PCR from the total RNA extracted from peripheral blood lymphocytes,and then cloned and sequenced.The result showed that the full length of TLR7 gene sequence from Sanyuan pig was consisted of 3 160 bp,which included one open-reading frame(3 153 bp),encoding 1 051 amino acid residues.There were fifteen potential N-glycosalation sites and four potential phosphorylation sites.The cleavage sites of signal peptide were predicted to be in 29th or 30th of the amino acid sequence,and the transmembrane helices in proteins was in 844-866th amino acid sequence.The results of comparative sequence analysis and phylogenetic tree analysis indicated that the TLR7 gene had difference of genus,and the nearer the genetic relationship,the higher homology.This study paved the way for future study of porcine TLR7 gene expression,biological function,mechanisms of infection with ssRNA viruses,antiviral immunity and breeding.