Antimicrobial Investigation of Datura stramonium Leaf Extract against different Microorganisms.

An extract of Datura stramonium was screened for phytochemical analysis and antimicrobial assay against different bacterial species. The solvent used for extract preparation was methanol. Phytochemical analysis of leaf extract shows presence of Alkaloids, phenols, carbohydrates, glycosides, Terpenoids, steroids and proteins. Agar well diffusion method was used for study of antimicrobial activity of leaf extract. Standard antibiotic (Azithromycin) and methanol were used as a positive and negative respectively. Leaf extract of Datura stramonium shows strong antimicrobial activity against bacterial species like Bacillus thuringiensis, Pseudomonas aeruginosa, Agrobacterium tumefaciens and Klebsiella pneumoniae.