Structural analysis of nonradioactive rna by postlabeling: The primary structure of baker's yeast tRNACUALeu

Summary The sequence of tRNA CUA Leu from baker's yeast was determined by application of novel postlabeling methods. Oligonucleotides in complete and partial RNase digests (A, T 1 , and U 2 ) were resolved by chromatography on polyethyleneimine-cellulose thin layers. Nucleotide sequences were elucidated by NaIO 4 /phosphatase or snake venom phosphodiesterase/phosphatase digestion and tritium postlabeling. A ribose-methylated constituent (Gm) was identified by 5′-terminal [ 32 P]-labeling. tRNA CUA Leu is the first biological macromolecule to be sequenced according to a postlabeling scheme.