Interleukine production in culture of mesenchymal stromal cells of humans during simulation of the microgravity effects

: Effects of simulated microgravity (cell clinostatting and containment in the Random Positioning Machine (RPM), Dutch Space, The Netherlands) on interleukins production by mesenchymal stromal cells (MSC) of the human marrow and MSC osteogenous derivatives obtained through cell stimulation by growth factors (10(-8) M dexamethazone, 0.2 mM ascorbic acid, 10 mM beta-glycerol-phosphate) were studied. Twenty-day clinostatting was found to increase 1.4 to 3.2 times the interleukin-8 (IL-8) content in the MSC and MSC osteogenous derivatives culture medium. Microgravity effects simulated with the use of RPM raised the IL-8 production 1.5 - 6 times and 1.6-2.1 times on the average after 10 days and 20 days of containment, respectively. MSC and MSC osteogenous derivatives demonstrated a downward trend in IL-6 secretion in the culture put in RPM. Therefore, simulation of the microgravity effects using different systems modifies interleukins production by MSC and also by mature cells of the osteoblastic phenotype.