Cyclic AMP in Leukemic Cell Lysate in Disseminated Intravascular Coagulation

Cyclic AMP (cAMP) and cyclic GMP contents were measured in 63 leukemic cell lysate from patients with and without disseminated intravascular coagulation (DIC) and compared with tissue factor (TF) activity, plasminogen activator (PA) and PA inhibitor (PAI). The cAMP level was increased in acute promyelocytic leukemia and was sighificantly higher in the leukemic cell lysate from patients with DIC than in from those without DIC. The cAMP level was well correlated with TF activity in the leukemic cell lysate. There was no difference in the phosphodiesterase level between the leukemic cell lysate from patients with DIC and from those without DIC, but calmodulin and s-100 levels were significantly higher in the former. The calmodulin level was also negatively correlated with the cAMP level and TF activity in leukemic cell lysates.It is speculated that cAMP may not be important in TF synthesis of leukemic cells and that calcium might regulate such synthesis.