Antibacterial Activity of Dadangkak Root Extract (Hydrolea spinosa L.) Against Propionibacterium acnes Bacteria

Women are prone to acne and they often treat it with antibiotics. However, there is a negative impact from the use of antibiotics, namely resistance and the incidence of antibiotic resistance against Propionibacterium acnes in Indonesia is quite high. This is the reason for the use of compounds from the Dadangkak plant ( Hydrolea spinosa L .) which are often used by people in South Kalimantan as treatment, especially in the root part which will be used as an antibacterial in Propionibacterium acnes . This study aims to determine the antibacterial activity of Dadangkak root extract against Propionibacterium acnes using the diffusion method and the dilution method to determine the value of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract. Testing the antibacterial activity of the extract of Dadangkak root ( Hydrolea spinosa L .) against the bacteria Propionibacterium acnes using the disc diffusion method as a screening test and the dilution method for testing MIC and MBC with concentrations of 20%, 40%, 60%, 80%, and 100%. 1% Clindamycin was used as a positive control and DMSO was used as a negative control. The results showed that the extract of Dadangkak root ( Hydrolea spinosa L .) had antibacterial activity which was indicated by the presence of a clear area around the disc and the MIC value at a concentration of 60% (0.06 g/ml) but no MBC value available. Based on the results of the research, it can be concluded that the extract of dadangak root has the potential as an antibacterial against bacteria Propionibacterium acnes with a Minimum Inhibitory Concentration value of 0,06 g/ml ( p value in Kruskall Wallis = 0,018 and p value in Post Hoc Mann-Whitney test = 0,025)