Improvement of restriction endonuclease cleavage at the termini of PCR products. Application to the Human Papillomavirus type 16 E7 gene

A protocol is described for the evaluation of the cleavage efficiency of the Human Papillomavirus (HPV) type 16 E7 oncogene synthetic ends by restriction endonucleasesBamHI andNdeI. which constitutes a quick and easy mean of optimizing subsequent ligation reactions. The syntheticBamHI site is located at two base pairs from one end, whereas theNdeI site is located four base pairs from the second E7 synthetic gene end.