Molecular plant biology : a practical approach

PART 1: GENE IDENTIFICATION 1. Classical mutagenesis in higher plants 2. Plant transfomrmation 3. T-DNA tagging 4. Transposion tagging methods in heterologous plants 5. Cloning plant genes by genomic subtraction PART 2: GENE ORGANIZATION 6. Approaches to gene mapping 7. Arabidopsis YAC, BAC and cosmid libraries 8. In situ hybridization to plant chromosomes and DNA fibres PART 3: LIBRARY SCREENING AND CDNA ISOLATION 9. PCR cloning approaches 10. Western and southwestern library screens 11. Complementation Cloning