PCC-FISH in Skin Fibroblasts for Local Dose Assessment: Biodosimetric Analysis of a Victim of the Georgian Radiological Accident

Abstract Pouget, J-P., Laurent, C., Delbos, M., Benderitter, M., Clairand, I., Trompier, F., Stephanazzi, J., Carsin, H., Lambert, F., Voisin, P. and Gourmelon, P. PCC-FISH in Skin Fibroblasts for Local Dose Assessment: Biodosimetric Analysis of a Victim of the Georgian Radiological Accident. Radiat. Res. 162, 365–376 (2004). We propose a new method of biodosimetry that could be applied in cases of localized irradiation. The approach is based on excess chromosome segments determination by the PCC-FISH technique in fibroblasts isolated from skin biopsy. Typically, 0 to 10 Gy ex vivo γ-irradiated human skin biopsies were dissociated and fibroblasts were isolated and grown for several days. Cells next underwent PCC-FISH painting of whole chromosome 4, and the number of excess chromosome segments per metaphase was determined. An ex vivo reference curve correlating the number of excess chromosome segments per metaphase to the radiation dose was established and used to assess the dose delivered to the skin of o...