歯周疾患と Histamine との関係

The histamine contents in whole saliva and gingival fluid collected from patients with periodontal disease were measured fluorometrically by Anton-Sayre's method, and compared with various clinical findings such as PMA-Index, OHI-score (debris score+calculus score), pocket depth (mm) and bone loss (%). The gingival fluid was collected from gingival sulcus by paper strips.The following results were obtained;1. Anton-Sayre's fluorometric method was able to measure histamine in concentrations from 0.05 to 10.0μg/ml.2. The histamine contents in whole saliva were showen to have marked correlations with pocket depth (P<0.001) and bone loss (P<0.001), but no significant correlation was found with other clinical findings.3. The histamine contents in gingival fluid from all clinically healthy subjects were able to be measured.4. Statistically significant correlations were showen between histamine contents in gingival fluid and following clinical findings; pocket depth (P<0.001), bone loss (P<0.001) and fluid quantity (P<0.001), except PMA-Index and OHI-score.5. A statistically significant correlation was showen between histamine contents in whole saliva and gingival fluid (P<0.001).6. In the group of patients that were examined on the basis of clinical findings and X-ray photos, the histamine contents in whole saliva and gingival fluid had a tendency to increase according to the progression of periodontal disease.It is concluded from these results that the histamine contents in whole saliva and gingival fluid are closely to the occurence and process of periodontal disease.