Perioperative oral supplementation with fish oil promotes liver regeneration following partial hepatectomy in mice via AMPK activation

: The present study aimed to observe the effects of perioperative oral supplementation with fish oil (FO) on liver regeneration in mice and examine the potential mechanism. A total of 120 male ICR mice were randomly divided into 5 groups: Sham, Control, fish oil (FO), Compound C [the AMP‑activated protein kinase (AMPK) inhibitor dorsomorphin], and Compound C + FO. Changes in liver function, alterations in hepatocyte proliferation and in the expression of polarization markers, and activation of AMPK signaling were examined following partial hepatectomy (PH). The results demonstrated that restoration of serum alanine aminotransferase (ALT) and total bilirubin (TBIL) levels were significantly faster in FO‑treated mice compared with Control mice, and this effect was suppressed by treatment with Compound C. FO‑treated mice exhibited increased numbers of Ki‑67 positive hepatocytes and their postoperative liver‑to‑body weight ratio was significantly increased compared with the Control mice, which was also suppressed by co‑treatment with the AMPK inhibitor. Furthermore, protein expression of Occludin, Claudin‑3, tight junction protein 1 and bile salt export pump was gradually increased in FO‑treated mice compared with Control, whereas Compound C treatment reversed this effect. Therefore, the present study revealed that perioperative oral supplementation with FO may promote liver regeneration and improved liver function in mice following PH through AMPK activation.