Anti lipid peroxidative and hypolipidaemic potentials of Nauclea latifolia leaves extract on ciprofloxacin induced oxidative stress in rats

Nauclea latifolia is commonly used by herbalist to cure so many diseases in Africa. There is little or no published information on the anti lipid peroxidative and hypolipidaemic potentials of N. latifolia, hence the need for this research. Eighty male and female Wister rats weighing 200g-300g were used. The rats were divided into four groups of twenty animals each. Group 1(Control) received normal saline 0.9% infusion w/v 5ml/kg body weight once daily. Group 2: received 70 mg/kg body weight of Ciprofloxacin, once daily. Group 3: received 70 mg/kg body weight of Ciprofloxacin and 1200mg/kg body weight of methanol extract of N. latifolia once daily. Group 4: received 70 mg/kg body weight of Ciprofloxacin, 2000mg/kg body weight of methanol extract of N. latifolia once daily. After 14 days of treatment, Twenty four hours after the last doses were administered, the animals were sacrificed. Blood was collected after fasting for 16 h. Lipid profile was estimated by colorimetric method and malondialdehyde by Standard curve method. Serum lipid profile level was measured ; total cholesterol, low density lipoprotein cholesterol and triacylglycerol (150.32±15.06 ; 107.07±7.99; 148.07±4.32)in ciprofloxacin treated rats significantly increased (P <0.005) while high density lipoprotein cholesterol(31.48±2.09), significantly decreased(P <0.005) when compared with the normal control(45.27±3.67). On administration of methanol extract of Nauclea latifolia leaf at doses of 1200mg/kg and 2000mg/kg body weight to rats treated with ciprofloxacin 70mg/kg/day body weight for 14 days, the serum lipid profile level in ciprofloxacin treated rats significantly decreased (P <0.005) while high density lipoprotein cholesterol, significantly increased when compared with the control after treatment with the extract. Also plasma and liver lipid peroxidation marker (malondialdehyde, MDA) was increased ( 6.25±0.69, 13.43±0.96) when compared with the controls (2.99±0.08 and 5.96±0.71).Ciprofloxacin can induce oxidative damage in rats and methanol extract of Nauclea latifolia leaf exhibits strong hypolipidaemic and antilipid peroxidative potentials. ACCESSION #