ANALYSIS OF MITOCHONDRIAL MEMBRANE POTENTIAL IN THROMBOCYTES FROM PARKINSON’S DISEASE PATIENTS
2015
Objectives So far there is no consensus on the physiological state of mitochondria in idiopathic Parkinson’s disease (IPD). Studies on the activity of respiratory chain complexes produced conflicting data. In this study we considered mitochondrial membrane potential as indicator for overall mitochondrial fitness in thrombocytes. Methodology 25 patients and 16 age matched healthy controls were recruited. Thrombocytes were purified using magnetic-activated cell sorting. Mitochondria with active mitochondrial membrane potential were stained with TMRM and single cell data was acquired via flow cytometry. After base line measurements, the cells were exposed to the protonophore FCCP and the fate of challenged cells analyzed over time. Results All study participants presented intact mitochondrial membrane potential and a significant reduction of mitochondrial membrane potential upon FCCP challenge (p < 0.001). However, no significant differences were found between patients and controls. Conclusion While the presented FCCP challenge assay is a valid marker of overall mitochondrial function, intact mitochondrial membrane potential in platelets derived from IPD patients suggests that presumed mitochondrial enzymatic deficiencies are compensated. In consequence, mitochondrial membrane potential in platelets cannot serve as diagnostic biomarker for non-stratified IPD. However, potential differences between disease subtypes are not excluded and should be further explored.
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