Synthesis of a C-Terminal Fluorescein-Labeled 36-mer Peptide

Biomedical researchers routinely incorporate fluorescently labeled peptides into assays to enhance detection sensitivity and/or to study receptor binding mechanisms associated with cellular physiology. While there are many methods available that allow a researcher to fluorescently label a peptide at the N-terminus, or on a lysine residue, there are no strategies for C-terminal labeling with fluorescein derivatives that utilize the carboxylic acid of amino acids [1,2].