Co-culture 3T3 fibroblast and macrophage on blood fibrin clot with rhbFGF

Aim: To investigate the behaviour of 3T3 fibroblast and macrophage co-culture on blood fibrin clot with recombinant basic fibroblast growth factor (rhbFGF). Methods: Using MTT method, inverted contrast microscopy, Giemsa staining as well as scanning electron microscope. Results: the effect of rhbFGF on co-culture of 3T3 fibroblast and mouse macrophage on blood fibrin clot in low-serum DMEM with rhbFGF were monitored, and 3T3 fibroblast and macrophage growed well on the blood fibrin blot in low-serum DMEM with rhbFGF. Conclusion: The blood fibrin clot, a low immunogenicity or non-antigen, could be used as a bionic support for three-dimensional tissue culture, and also a physiological carrier to distribute the growth factor rhbFGF for the cells. [