Effectivity of Nano herbal Andaliman (Zanthoxylum acanthopodium) to the Vascular Endothelial Growth Factor (VEGF) expression in burn wound in diabetic rats

Diabetes mellitus (DM), commonly known as a metabolic disease, is characterized by hyperglycemia caused by the inability of the pancreas to secrete the hormone insulin, impaired insulin action, or both. Hyperglycemia will usually increase Reactive Oxidative Stress and trigger oxidative stress so that it can interfere with every phase of wound healing. Vascular Endothelial Growth Factor (VEGF) plays a role in the angiogenesis process in providing nutrition to wound tissue. One of the natural herbal plants that has the ability to heal burns in DM patients is Andaliman (Zanthoxylum acanthopodium). This is because Andaliman contains terpenoids, flavonoids, tannins, and saponins. This study aims to determine the effectiveness of Andaliman nano herbal against VEGF protein during the healing process of burns in diabetic rats. In this study, Experiments were designed into two groups of burn rats: untreated and treated with Andaliman nano herbal. Treatment and surgery were performed on the days of 0, 4, 8, 12, and 16, respectively. Furthermore, the VEGF protein expression using immunohistochemical staining was analyzed. The results showed (Kruskal-Wallis test) that administration with the Andaliman nano herbal was highly effective in increasing VEGF expression from the day of 4 to day of 16 (p<0.05, p=0.000). It can be concluded that the nano herbal Andaliman is highly effective in increasing the expression of VEGF during the healing process of burns in diabetic rats.