[Construction and expression of prokaryotic expression vector for pTAT-HBV targeted ribonuclease fusion protein].

AIM: To construct Tat-HBV targeted ribonuclease fusion protein prokaryotic expression vector and express it in E. coli. METHODS: The cDNAs encoding HBV targeted ribonuclease, human eosinophil-derived neurotoxin and HBV core protein were respectivly cloned into prokaryotic expression vector pTAT-HA. Recombinant plasmids were transformed into E. coli BL21 ( DE3) LysS, then the transformed cells were induced with IPTG. The expression of the fusion proteins were analyzed by SDS-PAGE and Western blot. RESULTS: The three recombinant plasmids were constructed and expressed after IPTG induction successfully. CONCLUSION: The obtained Tat-HBV targeted ribonuclease fusion protein has laid the foundation for using TR in therapy of HBV infection.