A kind of method and reagent that excretion body is extracted from urine
2017
The invention discloses from it is a kind of from urine separate excretion body method and reagent.The present invention provides following reagent:Polyethylene glycol and three (2 carboxyethyl) phosphine (TCEP) mixed solutions.The experimental method of the present invention includes successively:Urine is first subjected to centrifugal treating;Reagent of the present invention is mixed according to certain proportioning again;The reagent of mixing is added in urine according to certain ratio afterwards, mixed;4 DEG C, stand overnight;Next day, after centrifugal treating, supernatant is abandoned, gained precipitation is excretion body.It is an advantage of the current invention that the yield that urine excretion secretes body in vitro is high, purity is high, and simple to operate, cost is low.
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