Esterase Catalyzed Regio- and Enantio-selective Hydrolysis of Substituted Carboxylates

Optically active α-substituted carboxylic acid derivatives were obtained with regio- and enantio-selective hydrolysis catalyzed by esterase from Pseudomonas putida MR-2068. Substrate specificities were summarized in empirical rule to predict the enantiopreferences of the esterase. High regio-selectivities were also discussed with binding models.