Close interactions between sympathetic neural fibres and follicular dendritic cells network are not altered in Peyer’s patches and spleen of C57BL/6 mice during the preclinical stage of 139A scrapie infection

Abstract During preclinical stage of prion diseases, secondary lymphoid organs seem to play an important role in prion amplification prior the invasion of the associated peripheral nervous system. In mice, it was shown that the relative positioning of follicular dendritic cells (FDC) and sympathetic nervous system (SNS) affects the velocity of neuroinvasion following scrapie inoculation. In this study, we checked if scrapie infection, by oral or intraperitoneal route, could influence this neuroimmune interface between FDC and tyrosine hydroxylase (TH) positive neural fibres within Peyer’s patches (PP) and spleen of the C57BL/6 mouse strain. We concluded that, in vivo , scrapie 139A and ME7 strains do not modify FDC–SNS neuroimmune interface. However, age seems to alter this neuroimmune interface and thus could influence the neuroinvasion in prion pathogenesis.