Effects of antisense oligonucleotides on the expression of focal adhesion kinase gene and collagen synthesis in the cultured human fibroblasts of hypertrophic scar

Objective To study the role of focal adhesion kinase(FAK) in the pathogenesis of human hyperthrophic scar.Methods Human hyperthrophic scar flbroblasts (HSFB) were isolated from human hypertrophic scar and cultured in vitro.The cells were then divided into 3 groups as AT group(phosphorethioate FAK ASODN was transfectod into the HSFB by liposome),I.PC group( liposeme only),and LC group( control group,without liposome or ASODN).The FAKmRNA index of HSFB was assessed by polymerase chain reaction method( FQ-PCR).The collagen sythesis of HSFB was assessed by 3 H-proline incorporation method.Results The FAK mRNA index of HSFB in AT group 48 hours after trasfection was significantly lower than that in LPC and LC groups (0.043±0.030,0.124±0.070,0.127±0.0195,P＜0.05 ).The 3 H-proline incorporation rote in AT group was lower than that in LPC and LC groups(257.0±15.14,962.2±300.5,930.8±28.97,P ＜0.01).Conclusion The expression of FAK gene and collagen synthesis of the cultured HSFB could be inhibited by FAK ASODN,indicating that FAK played a role in the development of excessive fibrosis of human hypertrophic scar. Key words: Scars;  Fibroblasts;  FAK;  Oligonucleotides,antisense;  Collagen