Study of Antitumor Activities of Polysaccharides from Tricholoma matsutake in Vitro

The anti-tumor activity was assayed by MTT method together with morphological observation under inversed light microscope.The cytotoxic activities of different concentrations and different time of MTS in vitro were investigated against human tongue carcinoma cells Tca8113,human liver cancer cells HepG-2,human pancreatic cancer cells HS766T,human cervical cancer cells Hela and human breast cancer cells MCF-7.The results showed that the low concentration of polysaccharides from T.matsutake weakly inhibited the growth of HepG-2 and HS766T but obviously affected the growth of Tca8113,Hela and MCF-7.It showed potential antiproliferative activity with dose-dependent and time-dependent relation in vitro.Maximum inhibition ratios of MTS under the 100 μg·mL-1 and 72h to Tca8113,HepG-2,HS766T,Hela and MCF-7 were 67.71%,74.89%,83.83%,75.57%,76.15%,repectively.From the cell morphology observation,polysaccharides of T.matsutake can give rise to different degrees of apoptosis on the five tumor cells mentioned above,the mechanism needs further study.