Description of Aphelenchoides tabarestanensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus brutia in Northern Iran

Aphelenchoides tabarestanensis n. sp. was isolated during a survey of nematodes associated with bark samples of pine trees ( Pinus brutia ) in Mazandaran province, northern Iran. The new species is characterised by body length of 656 (580-786) μ m (females) and 490 (480-500)  μ m (males), weakly annulated cuticle, four lines in the lateral field, and an offset lip region. The stylet is 10.5 (10-11)  μ m long and has small basal swellings. The excretory pore is located at nerve ring level and the hemizonid was not seen. The post-vulval uterine sac is 40.8 (38-43)  μ m long, the spicules are relatively short (18-19  μ m in dorsal limb), and the male tail has the usual three pairs of caudal papillae (2 + 2 + 2). The female tail is subcylindrical with two or three minute processes at the tip. The new species belongs to the Group 3 category of Aphelenchoides species and is close to seven known species of the genus, namely: A. asteromucronatus , A. besseyi , A. fujianensis , A. goodeyi , A. ritzemabosi , A. siddiqii , and A. stellatus , but differs by body size, shape of tail mucron, stylet length, size of spicules, and length of post-vulval uterine sac. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rRNA supported the morphological results.