Investigation of retention behavior for racemate drugs on avidin- and modified avidin-column

Abstract The binding properties of a native avidin were investigated by recording the fluorescence spectra of the native avidin, and by measuring the avidin-drug binding and the hydrophobicty of drugs. Some emission intensities were diminished by mixing avidin and drugs solutions compared with the native avidin solution. Also, the Scatchard plot analysis were performed to evaluate the avidin-drug binding. Then, modified avidin columns were prepared by acylation of amino groups and carboxyl groups to examine the retention and enantioselective properties of drugs. As a result, these functional groups contributed to chiral recognition of the avidin column for some drugs. Based on the results in this study, the avidin column have multiple binding sites for chiral separation.