On issue of detection of limits of normal reaction of thrombocytes in testing of impedance agregometry

: The study was carried out to evaluate analytical characteristics of impedance agregometry technique and aspirin test. The sampling included 89 healthy volunteers i.e. 55 females and 34 males. The analysis of aggregation of thrombocytes was implemented using aggregometer Chrono Log 700,. The ADP in ultimate concentration of 5 mkM was used as inductor. The coefficients were calculated concerning analytical, intra- and inter-individual variation (CVA, CV1, and CVG accordingly), index of individuality and critical level of difference (RCV). The study established that ADP-aggreagtion of thrombocytes was significantly higher in females as compared with males. The necessity of stratification of population by gender for calculation of reference limits was established using method of criterion Z calculation (Z=3.6; Zcrit=1.3). The intra-individual and analytical variation of impedance aggregometry made up to 5.8% and 8.1% correspondingly. Overall, parameters of aggregation in whole blood are characterized by high degree of individuality (II<0.06) that limits application of reference intervals in agregometry for differentiating norm and pathology. The statistically reliable alteration of functional activity of thrombocytes can be considered only at change of amplitude of aggregation no less than up to 26.9% as comparing with previous test in particular individual.