Assay for detecting pheromones natriuretic peptide and brain headphone

An in vitro method of determining activation or inactivation of the hormone system of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), the method comprising detecting simultaneously in a single reading or result the presence or amount of prohormones of atrial natriuretic peptide and brain (proANP and proBNP) or fragments thereof in a sample, said method comprising: (I) contacting the sample with a first substance binding bi or oligo-specific that is capable of binding both: (a) (i) proANP (SEQ ID NO: 1), ANP (SEQ ID NO: 2) or NT-proANP (SEQ ID NO: 3): (ii) a homologous sequence having at least 70% identity with (i); or (iii) a fragment of (i) or (ii) having at least 6 amino acids in length; and (b) (i) proBNP (SEQ ID NO: 4), BNP (SEQ ID NO: 5) or NT-proBNP (SEQ ID NO: 6); (Ii) a homologous sequence having at least 70% identity to (i) or (iii) a fragment of (i) or (ii) having at least 6 amino acids in length; or (II) contacting the sample with -a agent containing: (a) (i) proANP (SEQ ID NO: 1), ANP (SEQ ID NO: 2) or NT-proANP (SEQ ID NO: 3) ; (Ii) a homologous sequence having at least 70% identity to (i); or (iii) a fragment of (i) or (ii) having at least 6 amino acids in length; and (b) (i) proBNP (SEQ ID NO: 4), BNP (SEQ ID NO: 5) or NT-proBNP (SEQ ID NO: 6); (Ii) a homologous sequence having at least 70% identity to (i) or (iii) a fragment of (i) or (ii) having at least 6 amino acids in length; -a first substance and binding which is capable of binding to: (a) (i) proANP (SEQ ID NO: 1), ANP (SEQ ID NO: 2) or NT-proANP (SEQ ID NO: 3); (Ii) a homologous sequence having at least 70% identity to (i); or (iii) a fragment of (i) or (ii) having at least 6 amino acids in length; and (b) (i) proBNP (SEQ ID NO: 4), BNP (SEQ ID NO. 5) or NT-proBNP (SEQ ID NO: 6); (Ii) a homologous sequence having at least 70% identity to (i); or (iii) a fragment of (i) or (ii) having at least 6 amino acids in length; and (c) the agent.