Effect of Cooling on Force-Frequency Relationship, Rest Potentiation, and Frequency-Dependent Acceleration of Relaxation in the Guinea Pig Myocardium
2021
While deep hypothermia is well known to lead to cardiac malfunction
up to circulatory arrest, mild hypothermia can prevent hypoxic damage
to the heart. Importantly, a large body of research on deep hypothermia
was carried out on rats and mice whose myocardium is significantly
different from the human. In the present work, we investigated the
effect of deep hypothermia on rhythmoinotropic phenomena in the
guinea pig (GP) whose myocardium is more alike the human. The force––frequency
relationship (FFR), effect of post-rest potentiation, and frequency-dependent
acceleration of relaxation (FDAR) were studied in GP right ventricular papillary muscles (PM)
within a range of 0.1–3.0 Hz at temperatures of 30, 20 and 10°C.
It was shown that a positive FFR, mediated mainly by the inward
Ca2+ current through the L-type Ca2+ channel, persists
when cooling to 10°C, suggesting that this mechanism retains its
activity even under deep hypothermia. The effect of post-rest potentiation
persists down to 20°C, while further cooling replaces potentiation
by depression. This may indicate that at 10°C, the functioning of
the sarcoplasmic reticulum is impaired, as manifested in the rest-induced
inversion of the post-rest potentiation effect. The effect of frequency-dependent
acceleration of the kinetics of muscular contraction also persists
down to 20°C, supporting the suggestion that this effect in the
GP myocardium relies on the sarcoplasmic reticulum. Thus, we found
that among the studied frequency-dependent effects, there are those
affected by deep hypothermia (post-rest potentiation effect, FDAR)
and those resistant to this exposure (FFR), which may reflect the
differences in thermal sensitivity of the underlying mechanisms
of Ca2 + transport.
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