MORPHOLOGY,STRUCTURE,GROWTH AND PROLIFERATION OF Ecs CO-CULTURED WITH SMCs
2000
Objective\ Effects of smooth muscle cells(SMCs)on morphology and function of endothelial cells (Ecs) has been studied in order to support the basic theory for tissue engineering. Methods\ A new co\|culture model of Ecs\|SMCs which mimicked the interior architecture of vessel and also imitated the interaction patterns between Ecs and SMCs was established. Morphological observation, cell calculating were applied in the research of morphology, proliferation of the Ecs. Results\ The results showed that the shape of co\|culturing Ecs tended to elongating, the micro\|filaments paralleling the cell membrane increased, the proliferation of the Ecs was inhibited. Conclusions\ The new co\|culture model of Ecs\|SMCs is the best one to reveal the interaction of the two kinds of the cells. The morphology of the co\|culture Ecs is more similar to that in vivo. The change of the Ecs is beneficial to regulating the functions stress\|resistance of the Ecs in co\|culture model.\;
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