Detection of Y chromosome in human and Microtus mandarinus with FISH

The purpose of the investigation was to detect the Y chromosome of human and Microtus mandarinus with the satellite DNA probe in human′s Yq by fluorescence in situ hybridization(FISH). We hybridizied the probe with the metaphase chromosome and interphase nucleus specimens of humanbeing and Microtus mandarinus respectively.The results showed that there was hybridization signal on metaphase chromosome and interphase nucleus of human,but no signal on metaphase chromosome and interphase nucleus of Microtus mandarinus.According to the results,we discussed the homologue between pY3.4 in Y chromosome of humanbeing and DNA of Microtus mandarinus and FISH result of Microtus mandarinus.