Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection of Toxoplasma gondii infections

Aref Teimouri,Mohammad Hossein Modarressi,Saeedeh Shojaee,Mehdi Mohebali,M Rezaian,Hossein Keshavarz

Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection of Toxoplasma gondii infections

2019

Aref Teimouri
Mohammad Hossein Modarressi
Saeedeh Shojaee
Mehdi Mohebali
M Rezaian
Hossein Keshavarz

Background The aim of the present study was to develop a simple, portable, and rapid assay for serodiagnosis of toxoplasmosis based on recombinant Toxoplasma gondii (T. gondii) SAG1 (rSAG1) and GRA7 (rGRA7) proteins.

Keywords:

Toxoplasma gondii Infections
Virology
Serology
Biology
dot elisa
Recombinant DNA
Antigen
Toxoplasmosis
Escherichia coli
Toxoplasma gondii
Affinity chromatography

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