Comparative transcriptome analysis of the hepatopancreas of Eriocheir sinensis following oral gavage with enrofloxacin

Enrofloxacin is an important drug that is widely used in the treatment of the diseased Eriocheir sinensis. This study compared transcriptome differences in the hepatopancreas of E. sinensis following oral gavage with enrofloxacin. Our study produced 80 228 728 and 88 888 706 raw reads from control and treatment groups, and after filtering and quality checks of the raw sequence reads, our analysis yielded 78 843 613 and 87 628 922 clean reads with a mean length of 126 bp from control and treatment groups, respectively. A total of 15 797 transcripts were assembled, with 11 975 transcripts annotated. Moreover, 2795 transcripts were judged to be differentially expressed genes. Gene ontology terms “biological process” and “metabolic process” were the most enriched in the oxidation–reduction process, translational initiation, membrane, cytoplasmic part, and hydrolase activity. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that metabolic and signal transduction pathways were significantly enric...