Effect of cyclodextrins on the stability of adriamycin, adriamycinol, adriamycinone and daunomycin

Abstract The stability of adriamycin (ADR), adriamycinol, adriamycinone (ADR-ONE) and daunomycin in the presence of α-, β- and γ-cyclodextrins (CDs) was studied using high-performance liquid chromatography. It was found that α-CD did not affect the degradation of tested compounds, β-CD caused a little effect and γ-CD resulted in pronounced stabilizing effect. The formation of complexes between ADR and ADR-ONE with CDs was monitored by fluorescence spectroscopy. The fluorescence spectrum of ADR-γ-CD complex had an activation maximum at 460 nm, emission maximum at 555 nm and a shoulder at 585 nm. A similar finding was observed in case of α-CD. In case of β-CD, the fluorescence intensity at 580 nm peak enhanced less than in case of γ-CD. With ADR-ONE, α-CD did not cause any significant change compared with the spectrum of free molecule. On the other hand, it was noticed that, the fluorescence spectra of ADR-ONE with both β- and γ-CD were the same but showed a significant difference to the spectrum of free molecule, especially the molar fluorescence of the 585 nm emission peak.