The effects of Isatis tinctoria extract on diseases resistance against Aeromonas hydrophila and pigmentation, growth of Pseudotropheus acei

This study was conducted with aim to investigate the effects of Isatis tinctoria leaves extract on growth performance, pigmentation and protection against Aeromonas hydrophila of Pseudotropheus acei. Five diets were prepared by adding four different ratios fish woad extraction (Woad: W1: 1%, W1.5: 1.5 %, W2: 2 %, W2.5: 2.5% and C: control diet 0 %). Three replicates were conducted for each treatment. One hundred twenty fish and 15 tanks (80 x 30 x 80 cm) were used in recirculating system. In the end of 90 days, there were no differences in terms of weight gain, FCR and SGR of Pseudotropheus acei feed with diets added with different levels of Isatis tinctoria extraction compare to control group. Isatis tinctoria extract was not found effect on survival rates in Pseudotropheus acei after A. hydrophila infection. However, there were no differences on pigmentation of Pseudotropheus acei. Isatis tinctoria extract administration did not provide protection against Aeromonas hydrophila infection.