Studies on the enzymatic hydrolysates antimicrobial activity of Mytilus edulis and optimal parameters of hydrolysis

Using fruit and vegetable pathogen Botryis cinerea as tested fungi, this research studied on the effects of mytilus edulis enzymatic hydrolysates with trypsin, pepsin, flavory protease and bromelin against Botryis cinerea in vitro, which was determined by 96-well microtiter plates incubation method. The hydrolysis conditions of the trypsin was also investigated by orthogonal test. The result indicated that the inhibition rate of trypsin hydrolysates was 38.4%, 19.5% and 18.2% higher than pepsin, flavory protease and bromelin hydrolysates, respectively. The results show that the optimal hydrolysis conditions of trypsin was 55℃, pH 8.5, enzyme dosage 300U/g , time 2h and substrate concentration(w/v)1∶1.