A New Benzophenone and Bioactive Constituents of Hypericum nokoense

A new benzophenone derivative, hyperinokone (1), has been isolated from the aerial part of Hypericum nokoense, together with six known compounds. The structure of the new compound 1 was determined through spectroscopic and MS analyses. Among the isolates, isojacareubin (4), 5,4′ -dihydroxy-3′ -methoxy-(6:7)-2,2-dimethylpyranoflavone (5), and eleocharin A (6) exhibited cytotoxicities, with IC50 values of 4.16, 6.28, and 5.98 μM, respectively, against the DLD-1 cell line. Compounds 4 and 6 exhibited cytotoxicities, with IC50 values of 5.24 and 4.79 μM, respectively, against the IMR-32 cell line. In addition, 3, 4, and 6 showed potent inhibition with IC50 values of 7.67, 6.03, and 10.52 μM, respectively, against LPS-induced NO generation.