Mid-infrared spectroscopy on skin using a silver halide fibre probe in vivo

Background/aim: Mid-infrared spectroscopy is a versatile method for in vivo investigation of skin after topical treatment with skin care products. Methods: FTIR-spectrometer (Bruker Optics) with a flexible silver halide fibre probe (Infrared Fiber Sensors). Results: Absorbance spectra from 700 to 3000 cm−1 have been recorded to gain information about proteins (amide-I and amide-II vibrations at 1650 and 1550 cm−1), esters (1740 cm−1), carboxylic acid (1710 cm−1), polyalcohols (1050 cm−1) and hydrocarbons (CHn vibrations at 2800–3000 cm−1). Conclusions: Using the particular light guide, we were able to measure for the first time the effects of lip care products on lips directly. Furthermore, water binding and glycerol content of the skin could be determined simultaneously, as well as the replenishment of lipids by lipid-enriched bath oil.